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In vitro efficacy of different antibiotics against contaminants of tissue - cultured ‘Lakatan’ banana

Magbalot-Fernandez, Alminda and Hindoy Jr., Carlito M. and Ubaub, Leslie (2021) In vitro efficacy of different antibiotics against contaminants of tissue - cultured ‘Lakatan’ banana. Annals of Tropical Research, 43 (2). pp. 35-50. ISSN 0116-0710

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This study aimed to characterize the bacterial and fungal contaminants of tissue-cultured 'Lakatan' banana ( ) and find out effective Musa acuminata antibiotics against these contaminants. This was conducted at the University of Southeastern Philippines, Tagum-Mabini Campus from October 2015 to February 2016. This experiment was laid out in a Completely Randomized Design (CRD) with five treatments replicated three times. The treatments were: T1-Control; T2- Streptomycin (200mg L ); T3-Nystatin (1mL L ); T4-Streptomycin (200mg L ) + -1 -1 -1 Nystatin (1mL L ); and T5- Benomyl (100mg L ). Data were analyzed using -1 -1 ANOVA and compared through HSD. Results showed that the different contaminants occurring during the initiation stage of tissue-cultured 'Lakatan' banana meriplants were composed of Rhizopus sp., an unidentified fungus, and Gram negative bacterium. Generally, 35% contamination was observed on this stage. The result revealed that colony diameter of unidentified fungus and Rhizopus sp. were inhibited by Nystatin (1mL L ). While the bacterial clear zone inhibition was increased by Streptomycin. This study elucidated the effectivity of antibiotics, nystatin and streptomycin against bacterial and fungal contaminants of tissue-cultured 'Lakatan' banana.

Item Type: Journal Article
Uncontrolled Keywords: Bacteria, Fungi, Contaminants, Tissue-culture, 'Lakatan' banana,Meriplants
Subjects: S Agriculture > S Agriculture (General)
S Agriculture > SB Plant culture
Divisions: School of Agriculture, Geography, Environment, Ocean and Natural Sciences (SAGEONS)
Depositing User: Leslie T Ubaub
Date Deposited: 18 Jan 2022 22:59
Last Modified: 18 Jan 2022 22:59

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