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Cryopreservation of in vitro grown shoot-tips of tropical taro (Colocasia esculenta var. esculenta) by vitrification

Sant, R. and Taylor, M. and Tyagi, Anand P. (2006) Cryopreservation of in vitro grown shoot-tips of tropical taro (Colocasia esculenta var. esculenta) by vitrification. Cryoletters, 27 (3). pp. 133-142. ISSN 0143-2044

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In vitro shoot-tips of three cultivars of tropical taro (Colocasia esculenta var. esculenta (L.) Schott) were successfully cryopreserved by vitrification. Different conditioning treatments were required for each of the cultivars, while the vitrification protocol was constant for all. For the cultivars E399 and CPUK, shoot-tips from three-month-old in vitro plants grown on solidified MS were preconditioned on MS with 0.3 M sucrose in the dark for 16 h at 25°C. For the cultivar TNS, donor plants were preconditioned on solid MS with 90 g L-1 sucrose for seven weeks before cryopreservation. For vitrification, the shoot-tips were loaded with a solution of 2 M glycerol plus 0.4 M sucrose for 20 min at 25°C, dehydrated with PVS2 for 12 min at 25°C and plunged in liquid nitrogen. Vials were warmed by rapid shaking in a water bath at 40°C for 1 min 30. Shoot-tips were rehydrated in liquid MS with 1.2 M sucrose for 15 min at 25°C then plated on recovery medium. Shoot-tips resumed growth within a week and developed into plantlets six to eight weeks later without any callus formation. The best mean recoveries for the three cultivars were 21, 29 and 30% for E399, CPUK and TNS, respectively. This protocol was evaluated with five other taro cultivars with no success. However, this study has shown that vitrification has potential for cryopreserving tropical taro.

Item Type: Journal Article
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Science, Technology and Environment (FSTE) > School of Biological and Chemical Sciences
Depositing User: Ms Neha Harakh
Date Deposited: 15 Mar 2006 03:40
Last Modified: 14 May 2012 05:47

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