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Minimizing fouling at hydrogenated conical - tip carbon electrodes during Dopamine detection in vivo

Chandra, Shaneel and Miller, A.D. and Bendavid, A. and Martin, P.J. and Wong, D.K.Y. (2014) Minimizing fouling at hydrogenated conical - tip carbon electrodes during Dopamine detection in vivo. Analytical Chemistry, 86 (5). pp. 2443-2450. ISSN 0003-2700

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    Abstract

    In this paper, physically small conical-tip carbon electrodes (∼2−5 μm diameter and ∼4 μm axial length) were hydrogenated to develop a probe capable of withstanding fouling during dopamine detection in vivo. Upon hydrogenation, the resultant hydrophobic sp3 carbon surface deters adsorption of amphiphilic lipids, proteins, and peptides present in extracellular fluid and hence minimizes electrode fouling. These hydrogenated carbon electrodes showed a 35% decrease in sensitivity but little change in the limit of detection for dopamine over a 7-day incubation in a synthetic laboratory solution containing 1.0% (v/v) caproic acid (a lipid), 0.1% (w/v) bovine serum albumin and 0.01% (w/v) cytochrome C (both are proteins), and 0.002% (w/ v) human fibrinopeptide B (a peptide). Subsequently, during dopamine detection in vivo, over 70% of the dopamine oxidation current remained after the first 30 min of a 60-min experiment, and at least 50% remained over the next half-period at the hydrogenated carbon electrodes. On the basis of these results, an initial average electrode surface fouling rate of 1.2% min−1 was estimated, which gradually declined to 0.7% min−1. These results support minimal fouling at hydrogenated carbon electrodes applied to dopamine detection in vivo.

    Item Type: Journal Article
    Additional Information: Analytical Chemistry
    Subjects: Q Science > QC Physics
    Q Science > QD Chemistry
    Divisions: Faculty of Science, Technology and Environment (FSTE) > School of Biological and Chemical Sciences
    Depositing User: Shaneel Chandra
    Date Deposited: 11 Sep 2015 09:57
    Last Modified: 05 May 2016 09:52
    URI: http://repository.usp.ac.fj/id/eprint/7265
    UNSPECIFIED

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