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Evaluation of the antioxidant activity of the marine pyrroloiminoquinone makaluvamines

Alonso, Eva and Alvariño, Rebeca and Leirós, Marta and Tabudravu, Jioji N. and Feussner, Klaus D. and Dam, Miriam A. and Rateb, Mostafa E. and Jaspars, Marcel and Botana, Luis M. (2016) Evaluation of the antioxidant activity of the marine pyrroloiminoquinone makaluvamines. Marine Drugs, 14 (11). pp. 197-209. ISSN 1660-3397

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    Abstract

    Makaluvamines are pyrroloiminoquinones isolated from Zyzzya sponges. Until now, they have been described as topoisomerase II inhibitors with cytotoxic effects in diverse tumor cell lines. In the present work, seven makaluvamines were tested in several antioxidant assays in primary cortical neurons and neuroblastoma cells. Among the alkaloids studied, makaluvamine J was the most active in all the assays. This compound was able to reduce the mitochondrial damage elicited by the well-known stressor H2O2. The antioxidant properties of makaluvamine J are related to an improvement of the endogenous antioxidant defenses of glutathione and catalase. SHSY5Y assays proved that this compound acts as a Nrf2 activator leading to an improvement of antioxidant defenses. A low concentration of 10 nM is able to reduce the reactive oxygen species release and maintain a correct mitochondrial function. Based on these results, non-substituted nitrogen in the pyrrole plus the presence of a p-hydroxystyryl without a double bond seems to be the most active structure with a complete antioxidant effect in neuronal cells.

    Item Type: Journal Article
    Subjects: Q Science > Q Science (General)
    Q Science > QD Chemistry
    Divisions: Faculty of Science, Technology and Environment (FSTE) > Institute of Applied Science
    Depositing User: Fulori Nainoca
    Date Deposited: 21 Feb 2017 13:25
    Last Modified: 04 Jul 2017 09:47
    URI: http://repository.usp.ac.fj/id/eprint/9618
    UNSPECIFIED

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