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Accurate quantification of modified cyclic peptides without the need for authentic standards

Adaba, Rosemary I. and Mann, Greg and Raab, Andrea and Houssen, Wael E. and McEwan, Andrew R. and Thomas, Louise and Tabudravu, Jioji N. and Naismith, James H. and Jaspars, Marcel (2016) Accurate quantification of modified cyclic peptides without the need for authentic standards. Tetrahedron, 72 (52). pp. 8603-8609. ISSN 0040-4020

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There is a growing interest in the use of cyclic peptides as therapeutics, but their efficient production is often the bottleneck in taking them forward in the development pipeline. We have recently developed a method to synthesise azole-containing cyclic peptides using enzymes derived from different cyanobactin biosynthetic pathways. Accurate quantification is crucial for calculation of the reaction yield and for the downstream biological testing of the products. In this study, we demonstrate the development and validation of two methods to accurately quantify these compounds in the reaction mixture and after purification. The first method involves the use of a HPLC coupled in parallel to an ESMS and an ICP-MS, hence correlating the calculated sulfur content to the amount of cyclic peptide. The second method is an NMR ERETIC method for quantifying the solution concentration of cyclic peptides. These methods make the quantification of new compounds much easier as there is no need for the use of authentic standards when they are not available.

Item Type: Journal Article
Subjects: Q Science > Q Science (General)
Divisions: Faculty of Science, Technology and Environment (FSTE) > School of Biological and Chemical Sciences
Depositing User: Fulori Nainoca - Waqairagata
Date Deposited: 17 May 2017 00:26
Last Modified: 05 Jun 2017 02:38

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