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Cryopreservation of shoot-tips by droplet vitrification applicable to all taro (Colocasia esculanta var. esculanta) accessions

Sant, R. and Panis, B. and Taylor, M. and Tyagi, Anand P. (2008) Cryopreservation of shoot-tips by droplet vitrification applicable to all taro (Colocasia esculanta var. esculanta) accessions. Plant Cell, Tissue and Organ Culture , 92 (1). pp. 107-111. ISSN 0167-6857

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Abstract

The application of the droplet vitrification cryopreservation technique to taro accessions from a range of Asia Pacific countries is presented. The optimum protocol involves excision of about 0.8 mm shoot-tips from in vitro plants, 20–40 min PVS2 exposure at 0°C followed by rapid plunge into liquid nitrogen. Thawing was done at room temperature (25°C) and shoot-tips inoculated on MS medium with 0.1 M sucrose regenerated into plantlets 4–6 weeks later. This new droplet vitrification protocol improved the mean post-thaw regeneration rates to 73–100% from 21–30% obtained with the previous cryo-vial vitrification protocol.

Item Type: Journal Article
Subjects: Q Science > QP Physiology
Divisions: Faculty of Science, Technology and Environment (FSTE) > School of Biological and Chemical Sciences
Depositing User: Ms Neha Harakh
Date Deposited: 11 Jan 2008 11:23
Last Modified: 18 Jul 2012 13:24
URI: http://repository.usp.ac.fj/id/eprint/212
UNSPECIFIED

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