Sant, R. and Panis, B. and Taylor, M. and Tyagi, Anand P. (2008) Cryopreservation of shoot-tips by droplet vitrification applicable to all taro (Colocasia esculanta var. esculanta) accessions. Plant Cell, Tissue and Organ Culture , 92 (1). pp. 107-111. ISSN 0167-6857
Full text not available from this repository.Abstract
The application of the droplet vitrification cryopreservation technique to taro accessions from a range of Asia Pacific countries is presented. The optimum protocol involves excision of about 0.8 mm shoot-tips from in vitro plants, 20–40 min PVS2 exposure at 0°C followed by rapid plunge into liquid nitrogen. Thawing was done at room temperature (25°C) and shoot-tips inoculated on MS medium with 0.1 M sucrose regenerated into plantlets 4–6 weeks later. This new droplet vitrification protocol improved the mean post-thaw regeneration rates to 73–100% from 21–30% obtained with the previous cryo-vial vitrification protocol.
| Item Type: | Journal Article |
|---|---|
| Subjects: | Q Science > QP Physiology |
| Divisions: | Faculty of Science, Technology and Environment (FSTE) > School of Biological and Chemical Sciences |
| Depositing User: | Ms Neha Harakh |
| Date Deposited: | 10 Jan 2008 23:23 |
| Last Modified: | 18 Jul 2012 01:24 |
| URI: | https://repository.usp.ac.fj/id/eprint/212 |
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