Maeaba, Wilson and Singh, Poonam and Prasad, Surendra (2025) Development of method for simultaneous determination of water - soluble vitamins (B2, B3, B6, B9 and C) in guava (Psidium guineense) fruit by HPLC-photodiode array detection. Journal of Food Composition and Analysis, 144 . NA. ISSN 0889-1575
Full text not available from this repository.Abstract
Water-soluble vitamins (WSVs) are essential nutrients for physiological and metabolic functions of the human body. The simultaneous determination of WSVs by high performance liquid chromatography (HPLC) has been a challenge for routine analysis due to poor vitamin-peak separations and lengthy retention times. The present paper reports the development of an analytical method for simultaneous separation of the five WSVs (B2, B3, B6, B9 and C) in guava (Psidium guineense) fruit, using an isocratic delivery HPLC photodiode array detection (PDA) system with less retention time. The method included optimization of appropriate wavelength, column, flow rate, acid modifier, mobile phase composition and pH. The concentrations (mg per 100 g)/retention times (minutes) for the studied WSVs in guava fruit were 277.6/2.38, 0.06/2.64, 0.06/3.01, 0.16/3.30 and 1.28/3.81 for vitamins C, B9, B2, B6 and B3, respectively. The optimum separations of WSVs standards and in sample solutions were achieved using the mobile phase composition of 20:80 % (methanol: trifluoroacetic acid), a C18 Nova-Pak Silica 110 A column (150-mm X 3.9 mm i.d., 5 µm) at 23°C with 0.5 mL/minute flow rate at wavelength 265 nm. All vitamins showed excellent separation just within 4 minutes retention time and exhibited excellent recovery (95.7–105 %), inter-and intraday precisions (0.70–2.67 % RSD) which displayed robustness, accuracy, and repeatability of the developed method.
| Item Type: | Journal Article |
|---|---|
| Subjects: | Q Science > QD Chemistry T Technology > TX Home economics |
| Divisions: | School of Agriculture, Geography, Environment, Ocean and Natural Sciences (SAGEONS) School of Pacific Arts, Communication and Education (SPACE) |
| Depositing User: | Ms Shalni Sanjana |
| Date Deposited: | 14 Jul 2025 00:41 |
| Last Modified: | 14 Jul 2025 00:41 |
| URI: | https://repository.usp.ac.fj/id/eprint/15090 |
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