Evaluation of physically small p-phenylacetate-modified carbon electrodes against fouling during dopamine detection in vivo

Chandra, Shaneel and Miller, A.D. and Wong, D.K.Y. (2013) Evaluation of physically small p-phenylacetate-modified carbon electrodes against fouling during dopamine detection in vivo. Electrochimica Acta, 101 . pp. 225-231. ISSN 0013-4686

PDF (Journal Article submitted 2012, published 2013) - Published Version Restricted to Repository staff only Download (689kB)

Abstract

In this paper, the effectiveness of anionic p-phenylacetate film was evaluated in protecting physically small conical-tip carbon electrodes (∼2�m radius and ∼4�m axial length)from fouling during dopamine detection in vivo. After characterising p-phenylacetate film-modified carbon electrodes in several redox systems in vitro, they were found to exhibit an 11% loss in dopamine oxidation signal over a 40-day storage period in ambient laboratory conditions, compared to over a 90% loss at bare carbon electrodes. In addition, by incubating in a synthetic laboratory solution containing the fouling reagents, 1.0% (v/v)caproic acid (a lipid), 0.1%(w/v) bovine serum albumin and 0.01%(w/v) cytochrome C (both are protein)and 0.002% (w/v) human fibrinopeptide B (a peptide), film-modified carbon electrodes showed a 29% reduction in the limit of detection and a 25%decrease in sensitivity for dopamine over 7 days, compared to undeterminable results arising from a severely degraded surface at bare carbon electrodes. During dopamine detection invivo,70- 95% of the dopamine oxidation current remained after the first 40min of the experiment, and at least 50% over the next 20min. In contrast, constant degradation in the dopamine oxidation signal was observed at bare carbon electrodes throughout the experiment. An average electrode surface fouling rate of 0.54%min−1 was estimated at the p-phenylacetate film-modified carbon electrodes during the first 40min of the experiments.

Item Type:	Journal Article
Subjects:	Q Science > QD Chemistry Q Science > QP Physiology
Divisions:	Faculty of Science, Technology and Environment (FSTE) > School of Biological and Chemical Sciences
Depositing User:	Shaneel Chandra
Date Deposited:	19 Jul 2013 02:25
Last Modified:	14 Jun 2016 03:15
URI:	https://repository.usp.ac.fj/id/eprint/5997

Actions (login required)

View Item