Magbalot-Fernandez, Alminda and Hindoy Jr., Carlito M. and Ubaub, Leslie (2021) In vitro efficacy of different antibiotics against contaminants of tissue - cultured ‘Lakatan’ banana. Annals of Tropical Research, 43 (2). pp. 35-50. ISSN 0116-0710
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Abstract
This study aimed to characterize the bacterial and fungal contaminants of tissue-cultured 'Lakatan' banana ( ) and find out effective Musa acuminata antibiotics against these contaminants. This was conducted at the University of
Southeastern Philippines, Tagum-Mabini Campus from October 2015 to February 2016.
This experiment was laid out in a Completely Randomized Design (CRD) with five treatments replicated three times. The treatments were: T1-Control; T2- Streptomycin (200mg L ); T3-Nystatin (1mL L ); T4-Streptomycin (200mg L ) + -1 -1 -1 Nystatin (1mL L ); and T5- Benomyl (100mg L ). Data were analyzed using -1 -1 ANOVA and compared through HSD.
Results showed that the different contaminants occurring during the initiation stage of tissue-cultured 'Lakatan' banana meriplants were composed of Rhizopus sp., an unidentified fungus, and Gram negative bacterium. Generally, 35% contamination was observed on this stage.
The result revealed that colony diameter of unidentified fungus and Rhizopus sp. were inhibited by Nystatin (1mL L ). While the bacterial clear zone inhibition was increased by Streptomycin. This study elucidated the effectivity
of antibiotics, nystatin and streptomycin against bacterial and fungal contaminants of tissue-cultured 'Lakatan' banana.
| Item Type: | Journal Article |
|---|---|
| Uncontrolled Keywords: | Bacteria, Fungi, Contaminants, Tissue-culture, 'Lakatan' banana,Meriplants |
| Subjects: | S Agriculture > S Agriculture (General) S Agriculture > SB Plant culture |
| Divisions: | School of Agriculture, Geography, Environment, Ocean and Natural Sciences (SAGEONS) |
| Depositing User: | Leslie T Ubaub |
| Date Deposited: | 18 Jan 2022 22:59 |
| Last Modified: | 18 Jan 2022 22:59 |
| URI: | https://repository.usp.ac.fj/id/eprint/13187 |
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